WebAug 7, 2024 · 1 Answer. [E::idx_find_and_load] Could not retrieve index file for 'gill.sorted.bam'. Means that you have not generated an index for a BAM file. This needs to be done for most downstream processing of BAM files: # generate index samtools index gill.sorted.bam # count genes htseq-count -f bam --strand=no gill.sorted.bam yyy.gtf > gill … WebSep 9, 2024 · 1 HISAT2官网下载 人类和小鼠的索引有现成的, HISAT2官网 可以直接下载进行序列比对。 如下图所示:选择hg19和mm10的index,文章中RNA-Seq测序数据,可以包括人类和小鼠的数据,因此需要小鼠和人类的索引。
「学转录组入门生信」如何为RNA-seq分析建立HISAT2索引_哔哩 …
WebHISAT2 graph-based alignment of next generation sequencing reads to a population of genomes HISAT2 is a fast and sensitive alignment program for mapping next-generation sequencing reads (both DNA and RNA) to a … WebMay 18, 2024 · Hisat2官网上人类基因组索引的下载 Hisat2是现在很流行的主流比对软件,在现实生活中的mRNA-seq中,有很多时候我们需要将拿到的转录组与参考基因组进行比对 。 与大多数比对软件一样,在进行比... HUBU生信 RNAseq 1.4 1.Module 1 - Introduction to RNA sequencing conanma tabix操作VCF文件 tabix 可以对NGS分析中常见格式的文件建 … finding dna matches
Hisat2官网上人类基因组索引的下载 - 简书
WebDec 1, 2024 · HISAT2是一款快速、敏感的序列比对软件。 使用改进的BWT算法,相比Bowtie/TopHat2具有更高的敏感性和更快的运算速度。 官网下载链接:http://www.psc.edu/user-resources/software/hisat2 安装HISAT2 我采用 conda 安装,也是最简单的方法 conda install -c bioconda hisat2 conda install -c bioconda/label/cf202401 … WebDescription. HISAT2 is a fast and sensitive alignment program for mapping next-generation sequencing reads (both DNA and RNA) to a population of human genomes as well as to a single reference genome. WebOverview. HISAT2 is a fast and sensitive alignment program for mapping next-generation sequencing reads (whole-genome, transcriptome, and exome sequencing data) to a … finding divisors of a number